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hCT3 AbTCR T cell caused significant regression of LAN1 neuroblastoma xenografts with low antigen density in mice (A) Cytolytic activity of CAR and AbTCR T cells against LAN1 tumor cells. n = 3. ∗ p < 0.05, ∗∗ p < 0.01 compared to CT3 CAR T cells; ## p < 0.01 compared to mock at the E:T ratios of 10:1 and 20:1. (B) IFN-γ levels in co-cultured supernatants with LAN1 cells at an E:T ratio of 10:1. n = 3. (C–E) Experimental schema (C), tumor volume (D), and Kaplan-Meier survival curve (E) of the LAN1 xenograft mouse model. n = 5. (F–H) Representative immunohistochemistry staining images for GPC2 (F) and CD3 (G) in tumor sections and corresponding quantification (H) from the LAN1 mouse model. Scale bars: 500 μm. (I) Immunohistochemical detection of CD4- and <t>CD8-positive</t> cells in tumor sections from LAN1 mouse model. Scale bars: 200 or 500 μm. (J and K) Representative immunofluorescence staining images (J) and corresponding quantification (K) in tumor sections from the LAN1 mouse model. Scale bars: 50 μm. (L and M) Multiplexed immunofluorescence in tumor sections from the LAN1 mouse model (L) and corresponding quantification results (M). Scale bars: 50 or 200 μm. Values represent mean ± SEM. ns p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01; ## p < 0.01. See also .
Anti Human Cd8, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hCT3 AbTCR T cell caused significant regression of LAN1 neuroblastoma xenografts with low antigen density in mice (A) Cytolytic activity of CAR and AbTCR T cells against LAN1 tumor cells. n = 3. ∗ p < 0.05, ∗∗ p < 0.01 compared to CT3 CAR T cells; ## p < 0.01 compared to mock at the E:T ratios of 10:1 and 20:1. (B) IFN-γ levels in co-cultured supernatants with LAN1 cells at an E:T ratio of 10:1. n = 3. (C–E) Experimental schema (C), tumor volume (D), and Kaplan-Meier survival curve (E) of the LAN1 xenograft mouse model. n = 5. (F–H) Representative immunohistochemistry staining images for GPC2 (F) and CD3 (G) in tumor sections and corresponding quantification (H) from the LAN1 mouse model. Scale bars: 500 μm. (I) Immunohistochemical detection of CD4- and <t>CD8-positive</t> cells in tumor sections from LAN1 mouse model. Scale bars: 200 or 500 μm. (J and K) Representative immunofluorescence staining images (J) and corresponding quantification (K) in tumor sections from the LAN1 mouse model. Scale bars: 50 μm. (L and M) Multiplexed immunofluorescence in tumor sections from the LAN1 mouse model (L) and corresponding quantification results (M). Scale bars: 50 or 200 μm. Values represent mean ± SEM. ns p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01; ## p < 0.01. See also .
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hCT3 AbTCR T cell caused significant regression of LAN1 neuroblastoma xenografts with low antigen density in mice (A) Cytolytic activity of CAR and AbTCR T cells against LAN1 tumor cells. n = 3. ∗ p < 0.05, ∗∗ p < 0.01 compared to CT3 CAR T cells; ## p < 0.01 compared to mock at the E:T ratios of 10:1 and 20:1. (B) IFN-γ levels in co-cultured supernatants with LAN1 cells at an E:T ratio of 10:1. n = 3. (C–E) Experimental schema (C), tumor volume (D), and Kaplan-Meier survival curve (E) of the LAN1 xenograft mouse model. n = 5. (F–H) Representative immunohistochemistry staining images for GPC2 (F) and CD3 (G) in tumor sections and corresponding quantification (H) from the LAN1 mouse model. Scale bars: 500 μm. (I) Immunohistochemical detection of CD4- and <t>CD8-positive</t> cells in tumor sections from LAN1 mouse model. Scale bars: 200 or 500 μm. (J and K) Representative immunofluorescence staining images (J) and corresponding quantification (K) in tumor sections from the LAN1 mouse model. Scale bars: 50 μm. (L and M) Multiplexed immunofluorescence in tumor sections from the LAN1 mouse model (L) and corresponding quantification results (M). Scale bars: 50 or 200 μm. Values represent mean ± SEM. ns p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01; ## p < 0.01. See also .
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hCT3 AbTCR T cell caused significant regression of LAN1 neuroblastoma xenografts with low antigen density in mice (A) Cytolytic activity of CAR and AbTCR T cells against LAN1 tumor cells. n = 3. ∗ p < 0.05, ∗∗ p < 0.01 compared to CT3 CAR T cells; ## p < 0.01 compared to mock at the E:T ratios of 10:1 and 20:1. (B) IFN-γ levels in co-cultured supernatants with LAN1 cells at an E:T ratio of 10:1. n = 3. (C–E) Experimental schema (C), tumor volume (D), and Kaplan-Meier survival curve (E) of the LAN1 xenograft mouse model. n = 5. (F–H) Representative immunohistochemistry staining images for GPC2 (F) and CD3 (G) in tumor sections and corresponding quantification (H) from the LAN1 mouse model. Scale bars: 500 μm. (I) Immunohistochemical detection of CD4- and <t>CD8-positive</t> cells in tumor sections from LAN1 mouse model. Scale bars: 200 or 500 μm. (J and K) Representative immunofluorescence staining images (J) and corresponding quantification (K) in tumor sections from the LAN1 mouse model. Scale bars: 50 μm. (L and M) Multiplexed immunofluorescence in tumor sections from the LAN1 mouse model (L) and corresponding quantification results (M). Scale bars: 50 or 200 μm. Values represent mean ± SEM. ns p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01; ## p < 0.01. See also .
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hCT3 AbTCR T cell caused significant regression of LAN1 neuroblastoma xenografts with low antigen density in mice (A) Cytolytic activity of CAR and AbTCR T cells against LAN1 tumor cells. n = 3. ∗ p < 0.05, ∗∗ p < 0.01 compared to CT3 CAR T cells; ## p < 0.01 compared to mock at the E:T ratios of 10:1 and 20:1. (B) IFN-γ levels in co-cultured supernatants with LAN1 cells at an E:T ratio of 10:1. n = 3. (C–E) Experimental schema (C), tumor volume (D), and Kaplan-Meier survival curve (E) of the LAN1 xenograft mouse model. n = 5. (F–H) Representative immunohistochemistry staining images for GPC2 (F) and CD3 (G) in tumor sections and corresponding quantification (H) from the LAN1 mouse model. Scale bars: 500 μm. (I) Immunohistochemical detection of CD4- and <t>CD8-positive</t> cells in tumor sections from LAN1 mouse model. Scale bars: 200 or 500 μm. (J and K) Representative immunofluorescence staining images (J) and corresponding quantification (K) in tumor sections from the LAN1 mouse model. Scale bars: 50 μm. (L and M) Multiplexed immunofluorescence in tumor sections from the LAN1 mouse model (L) and corresponding quantification results (M). Scale bars: 50 or 200 μm. Values represent mean ± SEM. ns p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01; ## p < 0.01. See also .
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hCT3 AbTCR T cell caused significant regression of LAN1 neuroblastoma xenografts with low antigen density in mice (A) Cytolytic activity of CAR and AbTCR T cells against LAN1 tumor cells. n = 3. ∗ p < 0.05, ∗∗ p < 0.01 compared to CT3 CAR T cells; ## p < 0.01 compared to mock at the E:T ratios of 10:1 and 20:1. (B) IFN-γ levels in co-cultured supernatants with LAN1 cells at an E:T ratio of 10:1. n = 3. (C–E) Experimental schema (C), tumor volume (D), and Kaplan-Meier survival curve (E) of the LAN1 xenograft mouse model. n = 5. (F–H) Representative immunohistochemistry staining images for GPC2 (F) and CD3 (G) in tumor sections and corresponding quantification (H) from the LAN1 mouse model. Scale bars: 500 μm. (I) Immunohistochemical detection of CD4- and CD8-positive cells in tumor sections from LAN1 mouse model. Scale bars: 200 or 500 μm. (J and K) Representative immunofluorescence staining images (J) and corresponding quantification (K) in tumor sections from the LAN1 mouse model. Scale bars: 50 μm. (L and M) Multiplexed immunofluorescence in tumor sections from the LAN1 mouse model (L) and corresponding quantification results (M). Scale bars: 50 or 200 μm. Values represent mean ± SEM. ns p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01; ## p < 0.01. See also .

Journal: Cell Reports Medicine

Article Title: Antibody-gamma/delta T cell receptors targeting GPC2 regress neuroblastoma with low antigen density

doi: 10.1016/j.xcrm.2025.102378

Figure Lengend Snippet: hCT3 AbTCR T cell caused significant regression of LAN1 neuroblastoma xenografts with low antigen density in mice (A) Cytolytic activity of CAR and AbTCR T cells against LAN1 tumor cells. n = 3. ∗ p < 0.05, ∗∗ p < 0.01 compared to CT3 CAR T cells; ## p < 0.01 compared to mock at the E:T ratios of 10:1 and 20:1. (B) IFN-γ levels in co-cultured supernatants with LAN1 cells at an E:T ratio of 10:1. n = 3. (C–E) Experimental schema (C), tumor volume (D), and Kaplan-Meier survival curve (E) of the LAN1 xenograft mouse model. n = 5. (F–H) Representative immunohistochemistry staining images for GPC2 (F) and CD3 (G) in tumor sections and corresponding quantification (H) from the LAN1 mouse model. Scale bars: 500 μm. (I) Immunohistochemical detection of CD4- and CD8-positive cells in tumor sections from LAN1 mouse model. Scale bars: 200 or 500 μm. (J and K) Representative immunofluorescence staining images (J) and corresponding quantification (K) in tumor sections from the LAN1 mouse model. Scale bars: 50 μm. (L and M) Multiplexed immunofluorescence in tumor sections from the LAN1 mouse model (L) and corresponding quantification results (M). Scale bars: 50 or 200 μm. Values represent mean ± SEM. ns p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01; ## p < 0.01. See also .

Article Snippet: Anti-human CD8 , CST , Cat#85336.

Techniques: Activity Assay, Cell Culture, Immunohistochemistry, Staining, Immunohistochemical staining, Immunofluorescence